Reconstitution
10 mg vial
B733

B7-33 10 mg

Educational recon guide for a 10 mg B7-33 (relaxin-mimetic) research vial and microgram-to-low-mg unit mapping. Research use only — no established human therapeutic dose.

Vial

10 mg

Your BAC water

2 mL

Concentration

5.00 mg/mL

Per unit

~50.0 mcg

Frequency

Once daily subcutaneous in secondary educational tables (study designs vary)

Route

Subcutaneous

Live reconstitution calculator

Defaults match this guide (10 mg + 2 mL BAC). Change water or dose and the unit tables below update automatically.

Step 1

Reconstitution

Peptide amount in the vial and how much bacteriostatic water you add.

Bacteriostatic water (mL)
Concentration5 mg/mL
Per 1 unit (0.01 mL)50 mcg
Per 10 units (0.1 mL)500 mcg

Step 2

Your dose

This guide uses microgram-scale research doses — quick picks are in mcg (100 mcg = 0.1 mg).

Desired dose

Quick select (mcg) — switch units or type any value

Insulin syringe (U-100)

Suggested: 30-unit barrel (smallest that fits this draw).

Your draw

Results

Total draw

4 units

Volume

0.040 mL

Doses per vial

50

Syringes needed

1

Barrel

30-unit

Fits one syringe?

Yes

30-unit · 0.3 mL

Filled to 4 u

Fill one 30-unit U-100 syringe to about 4 units (0.040 mL).

Research cycle phases

Units and volumes recompute from your Step 1 concentration (2 mL BAC → 5.00 mg/mL · 50.0 mcg/unit). Doses below are once daily during the course — not weekly.

Standard course

There is no validated human clinical dose for research-market B7-33. Preclinical work uses model-specific mg/kg schedules. Secondary educational charts for research vials often discuss roughly ~200–800 mcg/day up into low-milligram daily SC amounts — treat as recon math only.

Live from Step 1
PhaseDaily doseUnitsVolume
Days 1–14 (intro)200 mcg0.2 mg4 u0.04 mL200 mcg daily — lower educational SC example
Days 15–42 (standard educational)500 mcg0.5 mg10 u0.10 mL500 mcg daily — mid educational example

Alternate / higher courses

Shorter or higher daily courses discussed in research communities — not a clinical recommendation

Live from Step 1
PhaseDaily doseUnitsVolume
Higher educational · 1 mg daily × 281 mg1,000 mcg20 u0.20 mL1 mg daily — upper end of many community mcg→mg tables
Higher educational · 2 mg daily × 142 mg2,000 mcg40 u0.40 mLLow-mg daily example — still not a safety-endorsed human protocol

Supplies planner

Using the guide’s phase ladder (200 mcg → 500 mcg per injection (phase ladder)) over a 42-day course. Set a dose in Step 2 to plan supplies at your own amount.

Updates with calculator

Your recon

2 mL · 5.00 mg/mL

Dose in plan

Phase ladder

Syringe size

100-unit

Horizon

42-day course

B7-33 10 mg vials

2 vials

~16.8 mg mg total peptide over this 42-day course at 200 mcg → 500 mcg per injection (phase ladder). Each vial holds 10 mg.

Bacteriostatic water

2 × 3 mL or 1 × 10 mL

2 mL BAC per vial × 2 vials = 4.0 mL total. Buy as 2× 3 mL bottles or 1× 10 mL bottle (whichever you stock).

U-100 insulin syringes (100-unit)

42+ syringes

About one 100-unit syringe per injection for this schedule. Keep a few spares.

Alcohol swabs & sharps

~88 swabs · 1 sharps container

Wipe vial stopper and injection site each time. Dispose needles in a proper sharps container.

Reconstitution steps

  1. Allow the lyophilized 10 mg B7-33 vial and bacteriostatic water to reach room temperature.
  2. Draw 2.0 mL bacteriostatic water (default for this guide).
  3. Inject BAC slowly down the inner wall of the vial — avoid blasting the powder cake.
  4. Gently swirl or roll until the solution is clear. Do not shake hard (foaming).
  5. Label with recon date and concentration (e.g. "5.0 mg/mL — 2 mL BAC").
  6. Refrigerate at 2–8 °C (35–46 °F), protected from light. Prefer using a reconstituted multi-dose vial within ~28 days with sterile technique.

Storage

Lyophilized

Keep dry powder cold and dark when possible (−20 °C / −4 °F preferred for long-term research stock).

Reconstituted

Refrigerate at 2–8 °C (35–46 °F). Avoid freeze–thaw of the working solution. Prefer finishing within ~28 days.

  • ·Write recon date and BAC volume on the label.
  • ·Protect from light during storage.
  • ·Discard if cloudy, particulates, or odd odor appear after reconstitution.

Protocol notes

  • ·B7-33 is a single-chain derivative of the H2 relaxin B-chain designed as an RXFP1-biased research tool in fibrosis and vascular models.
  • ·Calculator doses are milligrams: 0.2 mg = 200 mcg.
  • ·Preclinical mg/kg schedules are not naive human vial schedules.
  • ·Research use only.

Research context & considerations

B7-33 was developed as a simplified relaxin-family peptide analogue with anti-fibrotic and vascular research interest while aiming for simpler chemistry than full H2 relaxin. Human therapeutic dosing is not established; research powders are not approved drugs.

  • ·Cardiovascular / fluid-balance signaling is part of relaxin biology — high-caution experimental compound.
  • ·Not FDA-approved for any indication via research-vial channels.
  • ·Pregnancy and pediatric use are outside research-hobby scope.

Catalog match

B7-33

10mg x 10 vials · from $80

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