FOXO4-DRI 2 mg
Educational recon guide for a 2 mg FOXO4-DRI research vial and intermittent senolytic-style unit mapping. Research use only — no established human therapeutic dose.
Vial size
Same research phases — calculator defaults and unit math match the selected vial strength.
Vial
2 mg
Your BAC water
1 mL
Concentration
2.00 mg/mL
Per unit
~20.0 mcg
Frequency
Short multi-day pulses (then long off) in secondary longevity write-ups; not a daily year-round peptide
Route
Subcutaneous
Live reconstitution calculator
Defaults match this guide (2 mg + 1 mL BAC). Change water or dose and the unit tables below update automatically.
Step 1
Reconstitution
Peptide amount in the vial and how much bacteriostatic water you add.
Step 2
Your dose
Quick-select common research doses (includes 2.5 / 7.5 for dual-agonist ladders) or type any mg / mcg value.
Quick select (mg) — switch units or type any value
Suggested: 100-unit barrel (smallest that fits this draw).
Your draw
Results
Total draw
100 units
Volume
1 mL
Doses per vial
1
Syringes needed
1
Barrel
100-unit
Fits one syringe?
Yes
100-unit · 1.0 mL
Filled to 100 u
Fill one 100-unit U-100 syringe to about 100 units (1 mL).
Research cycle phases
Units and volumes recompute from your Step 1 concentration (1 mL BAC → 2.00 mg/mL · 20.0 mcg/unit). Doses below are once daily during the course — not weekly.
Standard course
There is no validated human FOXO4-DRI dose. Preclinical senolytic work used multi-mg/kg intermittent SC schedules in animals. Longevity-community educational pulses often discuss roughly multi-milligram subcutaneous amounts for a few consecutive days, then weeks/months off. Low-mcg daily tables circulating online are alternate recon math only and may not reflect preclinical exposure scale.
| Phase | Daily dose | Units | Volume |
|---|---|---|---|
| Pulse day · intro | 2 mg2,000 mcg | 100 u | 1.00 mL2 mg — lower educational pulse-day amount |
| Pulse days 1–3 (common community framing) | 2 mg2,000 mcg | 100 u | 1.00 mLMulti-mg × ~3 consecutive days then long off — educational only; scale to vial size |
Alternate / higher courses
Shorter or higher daily courses discussed in research communities — not a clinical recommendation
| Phase | Daily dose | Units | Volume |
|---|---|---|---|
| Higher pulse-day · 10 mg (if vial allows) | 2 mg2,000 mcg | 100 u | 1.00 mLUpper educational multi-mg pulse illustration — watch volume / split syringes |
| Alternate low daily · 500 mcg × 14 days | 500 mcg0.5 mg | 25 u | 0.25 mLLow-mcg daily table seen on some recon sites — alternate math only, not preferred preclinical scale |
Supplies planner
Using the guide’s phase ladder (2 mg → 2 mg per injection (phase ladder)) over a 4-day course. Set a dose in Step 2 to plan supplies at your own amount.
Your recon
1 mL · 2.00 mg/mL
Dose in plan
Phase ladder
Syringe size
100-unit
Horizon
4-day course
FOXO4-DRI 2 mg vials
4 vials
~8 mg mg total peptide over this 4-day course at 2 mg → 2 mg per injection (phase ladder). Each vial holds 2 mg.
Bacteriostatic water
2 × 3 mL or 1 × 10 mL
1 mL BAC per vial × 4 vials = 4.0 mL total. Buy as 2× 3 mL bottles or 1× 10 mL bottle (whichever you stock).
U-100 insulin syringes (100-unit)
4+ syringes
About one 100-unit syringe per injection for this schedule. Keep a few spares.
Alcohol swabs & sharps
~16 swabs · 1 sharps container
Wipe vial stopper and injection site each time. Dispose needles in a proper sharps container.
Reconstitution steps
- Allow the lyophilized 2 mg FOXO4-DRI vial and bacteriostatic water to reach room temperature.
- Draw 1.0 mL bacteriostatic water (default for this guide).
- Inject BAC slowly down the inner wall of the vial — avoid blasting the powder cake.
- Gently swirl or roll until the solution is clear. Do not shake hard (foaming).
- Label with recon date and concentration (e.g. "2.0 mg/mL — 1 mL BAC").
- Refrigerate at 2–8 °C (35–46 °F), protected from light. Prefer using a reconstituted multi-dose vial within ~28 days with sterile technique.
Storage
Lyophilized
Keep dry powder cold and dark when possible (−20 °C / −4 °F preferred for long-term research stock).
Reconstituted
Refrigerate at 2–8 °C (35–46 °F). Avoid freeze–thaw of the working solution. Prefer finishing within ~28 days.
- ·Write recon date and BAC volume on the label.
- ·Protect from light during storage.
- ·Discard if cloudy, particulates, or odd odor appear after reconstitution.
Protocol notes
- ·FOXO4-DRI is an experimental senolytic peptide (FOXO4–p53 disruption) with landmark mouse data and essentially no robust human dosing trials.
- ·Prefer intermittent pulse framing over continuous daily use in educational discussions.
- ·Research use only. Not a proven anti-aging medicine.
Research context & considerations
FOXO4-DRI (D-retro-inverso FOXO4 peptide) was designed to selectively clear senescent cells by disrupting FOXO4–p53 survival signaling. Preclinical work (e.g. Baar et al. lineage) is the primary evidence base; research powders are not approved senolytic drugs.
- ·Transient flu-like / apoptotic-cell clearance discussions appear in community reports after pulses.
- ·Unknown long-term human safety; high cost and supply quality risk.
- ·Active infection, serious illness, or pregnancy are outside research-hobby scope.
- ·Not FDA-approved.
Catalog match
FOX04 (F42)
2mg x 10 vials · from $70