Reconstitution
10 mg vial
HN10

Humanin 10 mg

Educational recon guide for a 10 mg Humanin research vial and milligram daily unit mapping. Research use only — no established human therapeutic dose.

Vial

10 mg

Your BAC water

2 mL

Concentration

5.00 mg/mL

Per unit

~50.0 mcg

Frequency

Once daily subcutaneous in secondary experimental tables

Route

Subcutaneous

Live reconstitution calculator

Defaults match this guide (10 mg + 2 mL BAC). Change water or dose and the unit tables below update automatically.

Step 1

Reconstitution

Peptide amount in the vial and how much bacteriostatic water you add.

Bacteriostatic water (mL)
Concentration5 mg/mL
Per 1 unit (0.01 mL)50 mcg
Per 10 units (0.1 mL)500 mcg

Step 2

Your dose

This guide uses microgram-scale research doses — quick picks are in mcg (100 mcg = 0.1 mg).

Desired dose

Quick select (mcg) — switch units or type any value

Insulin syringe (U-100)

Suggested: 30-unit barrel (smallest that fits this draw).

Your draw

Results

Total draw

10 units

Volume

0.100 mL

Doses per vial

20

Syringes needed

1

Barrel

30-unit

Fits one syringe?

Yes

30-unit · 0.3 mL

Filled to 10 u

Fill one 30-unit U-100 syringe to about 10 units (0.100 mL).

Research cycle phases

Units and volumes recompute from your Step 1 concentration (2 mL BAC → 5.00 mg/mL · 50.0 mcg/unit). Doses below are once daily during the course — not weekly.

Standard course

No validated human dose. Secondary experimental tables for humanin / HNG-class analogs often discuss roughly ~0.5–2 mg subcutaneous daily for multi-week research courses. Catalog “Humanin” may not equal potent analogs (e.g. HNG).

Live from Step 1
PhaseDaily doseUnitsVolume
Days 1–14 (intro)500 mcg0.5 mg10 u0.10 mL0.5 mg daily educational start
Days 15–42 (standard educational)1 mg1,000 mcg20 u0.20 mL1 mg daily

Alternate / higher courses

Shorter or higher daily courses discussed in research communities — not a clinical recommendation

Live from Step 1
PhaseDaily doseUnitsVolume
Higher educational · 2 mg × 28 days2 mg2,000 mcg40 u0.40 mLUpper common secondary experimental range

Supplies planner

Using the guide’s phase ladder (500 mcg → 1 mg per injection (phase ladder)) over a 42-day course. Set a dose in Step 2 to plan supplies at your own amount.

Updates with calculator

Your recon

2 mL · 5.00 mg/mL

Dose in plan

Phase ladder

Syringe size

100-unit

Horizon

42-day course

Humanin 10 mg vials

4 vials

~35 mg mg total peptide over this 42-day course at 500 mcg → 1 mg per injection (phase ladder). Each vial holds 10 mg.

Bacteriostatic water

3 × 3 mL or 1 × 10 mL

2 mL BAC per vial × 4 vials = 8.0 mL total. Buy as 3× 3 mL bottles or 1× 10 mL bottle (whichever you stock).

U-100 insulin syringes (100-unit)

42+ syringes

About one 100-unit syringe per injection for this schedule. Keep a few spares.

Alcohol swabs & sharps

~92 swabs · 1 sharps container

Wipe vial stopper and injection site each time. Dispose needles in a proper sharps container.

Reconstitution steps

  1. Allow the lyophilized 10 mg Humanin vial and bacteriostatic water to reach room temperature.
  2. Draw 2.0 mL bacteriostatic water (default for this guide).
  3. Inject BAC slowly down the inner wall of the vial — avoid blasting the powder cake.
  4. Gently swirl or roll until the solution is clear. Do not shake hard (foaming).
  5. Label with recon date and concentration (e.g. "5.0 mg/mL — 2 mL BAC").
  6. Refrigerate at 2–8 °C (35–46 °F), protected from light. Prefer using a reconstituted multi-dose vial within ~28 days with sterile technique.

Storage

Lyophilized

Keep dry powder cold and dark when possible (−20 °C / −4 °F preferred for long-term research stock).

Reconstituted

Refrigerate at 2–8 °C (35–46 °F). Avoid freeze–thaw of the working solution. Prefer finishing within ~28 days.

  • ·Write recon date and BAC volume on the label.
  • ·Protect from light during storage.
  • ·Discard if cloudy, particulates, or odd odor appear after reconstitution.

Protocol notes

  • ·Mitochondrial-derived peptide researched for cytoprotection / metabolism / aging models.
  • ·Human clinical dosing is not established.
  • ·Research use only.

Research context & considerations

Humanin is a mitochondrial-derived peptide with extensive preclinical literature. Research powders are not approved drugs; analogs (e.g. HNG) differ from native sequence products.

  • ·Unknown long-term human safety.
  • ·Not FDA-approved.
  • ·Pregnancy out of scope.

Catalog match

Humanin

10mg x 10 vials · from $65

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