ACE-031 1 mg
Educational recon guide for a 1 mg ACE-031 (myostatin-trap) research vial and infrequent-injection unit math. Research use only — clinical development was discontinued.
Vial
1 mg
Your BAC water
1 mL
Concentration
1.00 mg/mL
Per unit
~10.0 mcg
Frequency
Infrequent SC in literature (single dose or every 2–4 weeks in trials)
Route
Subcutaneous
Live reconstitution calculator
Defaults match this guide (1 mg + 1 mL BAC). Change water or dose and the unit tables below update automatically.
Step 1
Reconstitution
Peptide amount in the vial and how much bacteriostatic water you add.
Step 2
Your dose
This guide uses microgram-scale research doses — quick picks are in mcg (100 mcg = 0.1 mg).
Quick select (mcg) — switch units or type any value
Suggested: 30-unit barrel (smallest that fits this draw).
Your draw
Results
Total draw
25 units
Volume
0.250 mL
Doses per vial
4
Syringes needed
1
Barrel
30-unit
Fits one syringe?
Yes
30-unit · 0.3 mL
Filled to 25 u
Fill one 30-unit U-100 syringe to about 25 units (0.250 mL).
Weekly research phases
Units and volumes recompute from your Step 1 concentration (1 mL BAC → 1.00 mg/mL · 10.0 mcg/unit).
Standard titration
Published clinical work used body-weight doses (e.g. 0.02–3 mg/kg single SC). Community educational absolute tables often discuss ~0.25–1 mg per injection.
| Phase | Weekly dose | Units | Volume |
|---|---|---|---|
| Example · 0.25 mg | 250 mcg0.25 mg | 25 u | 0.25 mL≈25 units @ 1 mL recon |
| Example · 0.5 mg | 500 mcg0.5 mg | 50 u | 0.50 mL≈50 units @ 1 mL recon |
| Example · 1.0 mg (full vial) | 1 mg1,000 mcg | 100 u | 1.00 mL≈100 units — entire 1 mg vial |
Advanced / higher-end
Faster escalation discussed in research communities — not a clinical recommendation
| Phase | Weekly dose | Units | Volume |
|---|---|---|---|
| Compact recon note | 500 mcg0.5 mg | 50 u | 0.50 mLLower Step 1 water in the calculator to densify units for smaller absolute doses |
Supplies planner
Using the guide’s phase ladder (250 mcg → 500 mcg → 1 mg weekly (phase ladder)) over a 12-week schedule. Set a dose in Step 2 to plan supplies at your own amount.
Your recon
1 mL · 1.00 mg/mL
Dose in plan
Phase ladder
Syringe size
100-unit
Horizon
12-week schedule
ACE-031 1 mg vials
7 vials
~7 mg mg total peptide over this 12-week schedule at 250 mcg → 500 mcg → 1 mg weekly (phase ladder). Each vial holds 1 mg.
Bacteriostatic water
3 × 3 mL or 1 × 10 mL
1 mL BAC per vial × 7 vials = 7.0 mL total. Buy as 3× 3 mL bottles or 1× 10 mL bottle (whichever you stock).
U-100 insulin syringes (100-unit)
12+ syringes
About one 100-unit syringe per injection for this schedule. Keep a few spares.
Alcohol swabs & sharps
~38 swabs · 1 sharps container
Wipe vial stopper and injection site each time. Dispose needles in a proper sharps container.
Reconstitution steps
- Allow the lyophilized 1 mg ACE-031 vial and bacteriostatic water to reach room temperature.
- Draw 1.0 mL bacteriostatic water (default for this guide).
- Inject BAC slowly down the inner wall of the vial — avoid blasting the powder cake.
- Gently swirl or roll until the solution is clear. Do not shake hard (foaming).
- Label with recon date and concentration (e.g. "1.0 mg/mL — 1 mL BAC").
- Refrigerate at 2–8 °C (35–46 °F), protected from light. Prefer using a reconstituted multi-dose vial within ~28 days with sterile technique.
Storage
Lyophilized
Keep dry powder cold and dark when possible (−20 °C / −4 °F preferred for long-term research stock).
Reconstituted
Refrigerate at 2–8 °C (35–46 °F). Avoid freeze–thaw of the working solution. Prefer finishing within ~28 days.
- ·Write recon date and BAC volume on the label.
- ·Protect from light during storage.
- ·Discard if cloudy, particulates, or odd odor appear after reconstitution.
Protocol notes
- ·ACE-031 is a soluble activin type IIB receptor fusion protein studied as a myostatin/activin pathway trap.
- ·Clinical development was halted over safety concerns — recon math only.
- ·Research use only.
Research context & considerations
ACE-031 binds myostatin and related TGF-β superfamily ligands. Research-market powders are not approved therapeutics.
- ·Development stopped partly due to safety signals — high-caution compound.
- ·Not FDA-approved.
- ·Pregnancy and pediatric use are outside research-hobby scope.
Catalog match
ACE 031
1mg x 10 vials · from $80