Reconstitution
10 mg vial
ET10

Epithalon 10 mg

Educational guide for reconstituting a 10 mg Epithalon (Epitalon) research vial and mapping short daily cycle doses to U-100 units. Research use only.

Vial size

Same research phases — calculator defaults and unit math match the selected vial strength.

Vial

10 mg

Your BAC water

2 mL

Concentration

5.00 mg/mL

Per unit

~50.0 mcg

Frequency

Once daily for 10–20 consecutive days (then off)

Route

Subcutaneous (IM / nasal / sublingual discussed less often in secondary sources)

Live reconstitution calculator

Defaults match this guide (10 mg + 2 mL BAC). Change water or dose and the unit tables below update automatically.

Step 1

Reconstitution

Peptide amount in the vial and how much bacteriostatic water you add.

Bacteriostatic water (mL)
Concentration5 mg/mL
Per 1 unit (0.01 mL)50 mcg
Per 10 units (0.1 mL)500 mcg

Step 2

Your dose

Quick-select common research doses (includes 2.5 / 7.5 for dual-agonist ladders) or type any mg / mcg value.

Desired dose

Quick select (mg) — switch units or type any value

Insulin syringe (U-100)

Suggested: 50-unit barrel (smallest that fits this draw).

Your draw

Results

Total draw

50 units

Volume

0.500 mL

Doses per vial

4

Syringes needed

1

Barrel

50-unit

Fits one syringe?

Yes

50-unit · 0.5 mL

Filled to 50 u

Fill one 50-unit U-100 syringe to about 50 units (0.500 mL).

Research cycle phases

Units and volumes recompute from your Step 1 concentration (2 mL BAC → 5.00 mg/mL · 50.0 mcg/unit). Doses below are once daily during the course — not weekly.

Standard course

Common research-community range ~1–10 mg once daily for 10–20 day courses; many tables use ~5 mg/day, some clinic write-ups use ~10 mg nightly for 10 days. Cycles are often repeated only a few times per year after months off.

Live from Step 1
PhaseDaily doseUnitsVolume
Days 1–5 (optional intro)2.50 mg2,500 mcg50 u0.50 mLLower daily amount while checking injection-site tolerance
Days 6–20 (standard course)5 mg5,000 mcg100 u1.00 mL5 mg daily is a frequently cited research-community amount

Alternate / higher courses

Shorter or higher daily courses discussed in research communities — not a clinical recommendation

Live from Step 1
PhaseDaily doseUnitsVolume
Short course · 5 mg daily × 105 mg5,000 mcg100 u1.00 mL10 consecutive days at 5 mg — common shorter schedule
Higher daily · 10 mg × 10 days10 mg10,000 mcg200 u2.00 mL10 mg daily × 10 days — used in some secondary clinic write-ups (matches many 50 mg vial logistics)
Higher daily · 10 mg × 20 days10 mg10,000 mcg200 u2.00 mLUpper end of secondary dosage write-ups; plan multiple vials for smaller sizes

Supplies planner

Using the guide’s phase ladder (2.5 mg → 5 mg per injection (phase ladder)) over a 20-day course. Set a dose in Step 2 to plan supplies at your own amount.

Updates with calculator

Your recon

2 mL · 5.00 mg/mL

Dose in plan

Phase ladder

Syringe size

100-unit

Horizon

20-day course

Epithalon 10 mg vials

9 vials

~87.5 mg mg total peptide over this 20-day course at 2.5 mg → 5 mg per injection (phase ladder). Each vial holds 10 mg.

Bacteriostatic water

6 × 3 mL or 2 × 10 mL

2 mL BAC per vial × 9 vials = 18.0 mL total. Buy as 6× 3 mL bottles or 2× 10 mL bottles (whichever you stock).

U-100 insulin syringes (100-unit)

20+ syringes

About one 100-unit syringe per injection for this schedule. Keep a few spares.

Alcohol swabs & sharps

~58 swabs · 1 sharps container

Wipe vial stopper and injection site each time. Dispose needles in a proper sharps container.

Reconstitution steps

  1. Allow the lyophilized 10 mg Epithalon vial and bacteriostatic water to reach room temperature.
  2. Draw 2.0 mL bacteriostatic water (default). For denser draws, 1.0 mL is also used (10 mg/mL).
  3. Inject BAC slowly down the inner wall of the vial — avoid blasting the powder cake.
  4. Gently swirl or roll until the solution is clear. Do not shake hard (foaming).
  5. Label with recon date and concentration (e.g. "5.0 mg/mL — 2 mL BAC").
  6. Refrigerate at 2–8 °C (35–46 °F), protected from light. Prefer using a reconstituted vial within the short course window (~2–4 weeks).

Storage

Lyophilized

Keep dry powder cold and dark when possible (−20 °C / −4 °F preferred for long-term research stock).

Reconstituted

Refrigerate at 2–8 °C (35–46 °F). Avoid freeze–thaw of the working solution. Finish within the active course when practical.

  • ·Short courses mean most of a vial is used quickly — plan daily draws so leftover reconstituted solution is minimal.
  • ·Protect from light; pineal bioregulator peptides are often handled as light-sensitive research materials.
  • ·Write recon date and BAC volume on the label.

Protocol notes

  • ·This is a cyclic daily peptide in research discussions — not a once-weekly GLP-1-style titration.
  • ·Typical framing: inject once daily for 10 or 20 consecutive days, then stop for months.
  • ·Secondary sources often mention ~1–4 courses per year with long off intervals.
  • ·Rotate subcutaneous sites.
  • ·Research use only. Not a substitute for medical care.

Research context & considerations

Epithalon (also spelled Epitalon; sequence Ala-Glu-Asp-Gly) is a synthetic tetrapeptide associated with pineal bioregulator research. This page is a reconstitution and unit-math aid for research logistics only.

  • ·Injection-site irritation is the most practical day-to-day issue.
  • ·Not FDA-approved for any indication.
  • ·Active malignancy or serious endocrine disease should not be self-managed with research peptides.
  • ·Pregnancy, breastfeeding, and pediatric use are outside research-hobby scope.

Catalog match

Epithalon (ET10)

10mg x 10 vials · from $25

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