Epithalon 50 mg
Educational guide for reconstituting a 50 mg Epithalon (Epitalon) research vial and mapping short daily cycle doses to U-100 units. Research use only.
Vial size
Same research phases — calculator defaults and unit math match the selected vial strength.
Vial
50 mg
Your BAC water
5 mL
Concentration
10.00 mg/mL
Per unit
~100.0 mcg
Frequency
Once daily for 10–20 consecutive days (then off)
Route
Subcutaneous (IM / nasal / sublingual discussed less often in secondary sources)
Live reconstitution calculator
Defaults match this guide (50 mg + 5 mL BAC). Change water or dose and the unit tables below update automatically.
Step 1
Reconstitution
Peptide amount in the vial and how much bacteriostatic water you add.
Step 2
Your dose
Quick-select common research doses (includes 2.5 / 7.5 for dual-agonist ladders) or type any mg / mcg value.
Quick select (mg) — switch units or type any value
Suggested: 30-unit barrel (smallest that fits this draw).
Your draw
Results
Total draw
25 units
Volume
0.250 mL
Doses per vial
20
Syringes needed
1
Barrel
30-unit
Fits one syringe?
Yes
30-unit · 0.3 mL
Filled to 25 u
Fill one 30-unit U-100 syringe to about 25 units (0.250 mL).
Research cycle phases
Units and volumes recompute from your Step 1 concentration (5 mL BAC → 10.00 mg/mL · 100.0 mcg/unit). Doses below are once daily during the course — not weekly.
Standard course
Common research-community range ~1–10 mg once daily for 10–20 day courses; many tables use ~5 mg/day, some clinic write-ups use ~10 mg nightly for 10 days. Cycles are often repeated only a few times per year after months off.
| Phase | Daily dose | Units | Volume |
|---|---|---|---|
| Days 1–5 (optional intro) | 2.50 mg2,500 mcg | 25 u | 0.25 mLLower daily amount while checking injection-site tolerance |
| Days 6–20 (standard course) | 5 mg5,000 mcg | 50 u | 0.50 mL5 mg daily is a frequently cited research-community amount |
Alternate / higher courses
Shorter or higher daily courses discussed in research communities — not a clinical recommendation
| Phase | Daily dose | Units | Volume |
|---|---|---|---|
| Short course · 5 mg daily × 10 | 5 mg5,000 mcg | 50 u | 0.50 mL10 consecutive days at 5 mg — common shorter schedule |
| Higher daily · 10 mg × 10 days | 10 mg10,000 mcg | 100 u | 1.00 mL10 mg daily × 10 days — used in some secondary clinic write-ups (matches many 50 mg vial logistics) |
| Higher daily · 10 mg × 20 days | 10 mg10,000 mcg | 100 u | 1.00 mLUpper end of secondary dosage write-ups; plan multiple vials for smaller sizes |
Supplies planner
Using the guide’s phase ladder (2.5 mg → 5 mg per injection (phase ladder)) over a 20-day course. Set a dose in Step 2 to plan supplies at your own amount.
Your recon
5 mL · 10.00 mg/mL
Dose in plan
Phase ladder
Syringe size
100-unit
Horizon
20-day course
Epithalon 50 mg vials
2 vials
~87.5 mg mg total peptide over this 20-day course at 2.5 mg → 5 mg per injection (phase ladder). Each vial holds 50 mg.
Bacteriostatic water
4 × 3 mL or 1 × 10 mL
5 mL BAC per vial × 2 vials = 10.0 mL total. Buy as 4× 3 mL bottles or 1× 10 mL bottle (whichever you stock).
U-100 insulin syringes (100-unit)
20+ syringes
About one 100-unit syringe per injection for this schedule. Keep a few spares.
Alcohol swabs & sharps
~44 swabs · 1 sharps container
Wipe vial stopper and injection site each time. Dispose needles in a proper sharps container.
Reconstitution steps
- Allow the lyophilized 50 mg Epithalon vial and bacteriostatic water to reach room temperature.
- Draw 5.0 mL bacteriostatic water (default). For denser draws, 2.5–4.0 mL is also used (higher mg/mL, fewer units per mg).
- Inject BAC slowly down the inner wall of the vial — avoid blasting the powder cake.
- Gently swirl or roll until the solution is clear. Do not shake hard (foaming).
- Label with recon date and concentration (e.g. "10.0 mg/mL — 5 mL BAC").
- Refrigerate at 2–8 °C (35–46 °F), protected from light. Prefer using a reconstituted vial within the short course window (~2–4 weeks).
Storage
Lyophilized
Keep dry powder cold and dark when possible (−20 °C / −4 °F preferred for long-term research stock).
Reconstituted
Refrigerate at 2–8 °C (35–46 °F). Avoid freeze–thaw of the working solution. Finish within the active course when practical.
- ·Short courses mean most of a vial is used quickly — plan daily draws so leftover reconstituted solution is minimal.
- ·Protect from light; pineal bioregulator peptides are often handled as light-sensitive research materials.
- ·Write recon date and BAC volume on the label.
Protocol notes
- ·This is a cyclic daily peptide in research discussions — not a once-weekly GLP-1-style titration.
- ·Typical framing: inject once daily for 10 or 20 consecutive days, then stop for months.
- ·Secondary sources often mention ~1–4 courses per year with long off intervals.
- ·Rotate subcutaneous sites.
- ·Research use only. Not a substitute for medical care.
Research context & considerations
Epithalon (also spelled Epitalon; sequence Ala-Glu-Asp-Gly) is a synthetic tetrapeptide associated with pineal bioregulator research. This page is a reconstitution and unit-math aid for research logistics only.
- ·Injection-site irritation is the most practical day-to-day issue.
- ·Not FDA-approved for any indication.
- ·Active malignancy or serious endocrine disease should not be self-managed with research peptides.
- ·Pregnancy, breastfeeding, and pediatric use are outside research-hobby scope.
Catalog match
Epithalon (ET50)
50mg x 10 vials · from $60