PE-22-28 10 mg
Educational recon guide for a 10 mg PE-22-28 (spadin analog) research vial and microgram unit mapping. Research use only.
Vial
10 mg
Your BAC water
2 mL
Concentration
5.00 mg/mL
Per unit
~50.0 mcg
Frequency
Once daily subcutaneous (or intranasal in specialized formulations) for multi-week research courses
Route
Subcutaneous (intranasal discussed in some secondary write-ups)
Live reconstitution calculator
Defaults match this guide (10 mg + 2 mL BAC). Change water or dose and the unit tables below update automatically.
Step 1
Reconstitution
Peptide amount in the vial and how much bacteriostatic water you add.
Step 2
Your dose
This guide uses microgram-scale research doses — quick picks are in mcg (100 mcg = 0.1 mg).
Quick select (mcg) — switch units or type any value
Suggested: 30-unit barrel (smallest that fits this draw).
Your draw
Results
Total draw
1 units
Volume
0.010 mL
Doses per vial
200
Syringes needed
1
Barrel
30-unit
Fits one syringe?
Yes
30-unit · 0.3 mL
Filled to 1 u
Fill one 30-unit U-100 syringe to about 1 units (0.010 mL).
Research cycle phases
Units and volumes recompute from your Step 1 concentration (2 mL BAC → 5.00 mg/mL · 50.0 mcg/unit). Doses below are once daily during the course — not weekly.
Standard course
Secondary educational tables commonly discuss ~50–200 mcg once daily (sometimes up to ~500 mcg in higher write-ups). PE-22-28 is a TREK-1–related spadin analog studied for antidepressant/neuroplasticity signals in preclinical work — human dose not established.
| Phase | Daily dose | Units | Volume |
|---|---|---|---|
| Days 1–14 (intro) | 50 mcg0.05 mg | 1 u | 0.01 mL50 mcg daily — lower educational start |
| Days 15–56 (standard) | 100 mcg0.1 mg | 2 u | 0.02 mL100 mcg daily — mid common secondary range |
| Days 57–84 (optional) | 200 mcg0.2 mg | 4 u | 0.04 mL200 mcg daily — upper common educational range |
Alternate / higher courses
Shorter or higher daily courses discussed in research communities — not a clinical recommendation
| Phase | Daily dose | Units | Volume |
|---|---|---|---|
| Higher educational · 500 mcg × 28 days | 500 mcg0.5 mg | 10 u | 0.10 mLUpper secondary discussion range |
Supplies planner
Using the guide’s phase ladder (50 mcg → 100 mcg → 200 mcg per injection (phase ladder)) over a 84-day course. Set a dose in Step 2 to plan supplies at your own amount.
Your recon
2 mL · 5.00 mg/mL
Dose in plan
Phase ladder
Syringe size
100-unit
Horizon
84-day course
PE-22-28 10 mg vials
2 vials
~10.5 mg mg total peptide over this 84-day course at 50 mcg → 100 mcg → 200 mcg per injection (phase ladder). Each vial holds 10 mg.
Bacteriostatic water
2 × 3 mL or 1 × 10 mL
2 mL BAC per vial × 2 vials = 4.0 mL total. Buy as 2× 3 mL bottles or 1× 10 mL bottle (whichever you stock).
U-100 insulin syringes (100-unit)
84+ syringes
About one 100-unit syringe per injection for this schedule. Keep a few spares.
Alcohol swabs & sharps
~172 swabs · 1 sharps container
Wipe vial stopper and injection site each time. Dispose needles in a proper sharps container.
Reconstitution steps
- Allow the lyophilized 10 mg PE-22-28 vial and bacteriostatic water to reach room temperature.
- Draw 2.0 mL bacteriostatic water (default for this guide).
- Inject BAC slowly down the inner wall of the vial — avoid blasting the powder cake.
- Gently swirl or roll until the solution is clear. Do not shake hard (foaming).
- Label with recon date and concentration (e.g. "5.0 mg/mL — 2 mL BAC").
- Refrigerate at 2–8 °C (35–46 °F), protected from light. Prefer using a reconstituted multi-dose vial within ~28 days with sterile technique.
Storage
Lyophilized
Keep dry powder cold and dark when possible (−20 °C / −4 °F preferred for long-term research stock).
Reconstituted
Refrigerate at 2–8 °C (35–46 °F). Avoid freeze–thaw of the working solution. Prefer finishing within ~28 days.
- ·Write recon date and BAC volume on the label.
- ·Protect from light during storage.
- ·Discard if cloudy, particulates, or odd odor appear after reconstitution.
Protocol notes
- ·Spadin-optimized analog targeting TREK-1 potassium channel biology in preclinical depression models.
- ·Calculator: 0.1 mg = 100 mcg.
- ·Research use only.
Research context & considerations
PE-22-28 is derived from sortilin propeptide/spadin research on TREK-1 inhibition and neuroplasticity. Research vials are not approved antidepressants.
- ·CNS-active experimental peptide — unknown long-term human safety.
- ·Not FDA-approved.
- ·Pregnancy out of scope.
Catalog match
PE-22-28
10mg x 10 vials · from $25