Reconstitution
5 mg vial
PNC5

PNC-27 5 mg

Educational recon guide for a 5 mg PNC-27 research vial and microgram unit mapping. Research use only — experimental oncology peptide, not a wellness recovery product.

Vial

5 mg

Your BAC water

2 mL

Concentration

2.50 mg/mL

Per unit

~25.0 mcg

Frequency

Study-design dependent; some secondary sheets discuss multi-times-weekly microgram SC amounts

Route

Research-route dependent (secondary sheets often SC)

Live reconstitution calculator

Defaults match this guide (5 mg + 2 mL BAC). Change water or dose and the unit tables below update automatically.

Step 1

Reconstitution

Peptide amount in the vial and how much bacteriostatic water you add.

Bacteriostatic water (mL)
Concentration2.50 mg/mL
Per 1 unit (0.01 mL)25 mcg
Per 10 units (0.1 mL)250 mcg

Step 2

Your dose

This guide uses microgram-scale research doses — quick picks are in mcg (100 mcg = 0.1 mg).

Desired dose

Quick select (mcg) — switch units or type any value

Insulin syringe (U-100)

Suggested: 30-unit barrel (smallest that fits this draw).

Your draw

Results

Total draw

8 units

Volume

0.080 mL

Doses per vial

25

Syringes needed

1

Barrel

30-unit

Fits one syringe?

Yes

30-unit · 0.3 mL

Filled to 8 u

Fill one 30-unit U-100 syringe to about 8 units (0.080 mL).

Research cycle phases

Units and volumes recompute from your Step 1 concentration (2 mL BAC → 2.50 mg/mL · 25.0 mcg/unit). Doses below are once daily during the course — not weekly.

Standard course

PNC-27 is a chimeric p53/HDM-2–related membrane-active peptide studied for selective cancer-cell disruption in preclinical work. There is no approved human dose. Secondary research-clinic sheets sometimes list roughly ~200–300 mcg SC several times weekly — treat as recon math only, not cancer treatment.

Live from Step 1
PhaseDaily doseUnitsVolume
Example · 200 mcg200 mcg0.2 mg8 u0.08 mLLower secondary sheet amount
Example · 300 mcg300 mcg0.3 mg12 u0.12 mLMid secondary sheet amount

Alternate / higher courses

Shorter or higher daily courses discussed in research communities — not a clinical recommendation

Live from Step 1
PhaseDaily doseUnitsVolume
Example · 500 mcg500 mcg0.5 mg20 u0.20 mLHigher educational illustration only

Supplies planner

Using the guide’s phase ladder (200 mcg → 300 mcg per injection (phase ladder)) over a 2-day course. Set a dose in Step 2 to plan supplies at your own amount.

Updates with calculator

Your recon

2 mL · 2.50 mg/mL

Dose in plan

Phase ladder

Syringe size

100-unit

Horizon

2-day course

PNC-27 5 mg vials

1 vial

~500 mcg mg total peptide over this 2-day course at 200 mcg → 300 mcg per injection (phase ladder). Each vial holds 5 mg.

Bacteriostatic water

1 × 3 mL bottle

2 mL BAC per vial × 1 vial = 2.0 mL total. Buy as 1× 3 mL bottle or 1× 10 mL bottle (whichever you stock).

U-100 insulin syringes (100-unit)

2+ syringes

About one 100-unit syringe per injection for this schedule. Keep a few spares.

Alcohol swabs & sharps

~6 swabs · 1 sharps container

Wipe vial stopper and injection site each time. Dispose needles in a proper sharps container.

Reconstitution steps

  1. Allow the lyophilized 5 mg PNC-27 vial and bacteriostatic water to reach room temperature.
  2. Draw 2.0 mL bacteriostatic water (default for this guide).
  3. Inject BAC slowly down the inner wall of the vial — avoid blasting the powder cake.
  4. Gently swirl or roll until the solution is clear. Do not shake hard (foaming).
  5. Label with recon date and concentration (e.g. "2.5 mg/mL — 2 mL BAC").
  6. Refrigerate at 2–8 °C (35–46 °F), protected from light. Prefer using a reconstituted multi-dose vial within ~28 days with sterile technique.

Storage

Lyophilized

Keep dry powder cold and dark when possible (−20 °C / −4 °F preferred for long-term research stock).

Reconstituted

Refrigerate at 2–8 °C (35–46 °F). Avoid freeze–thaw of the working solution. Prefer finishing within ~28 days.

  • ·Write recon date and BAC volume on the label.
  • ·Protect from light during storage.
  • ·Discard if cloudy, particulates, or odd odor appear after reconstitution.

Protocol notes

  • ·Not a fat-loss, recovery, or anti-aging lifestyle peptide.
  • ·Calculator: 0.2 mg = 200 mcg.
  • ·Research use only — never a substitute for oncology care.

Research context & considerations

PNC-27 was designed to target HDM-2-expressing cancer cell membranes and induce selective membrane disruption in preclinical models. It is not an approved cancer therapy; research powders must not be used as DIY chemotherapy.

  • ·Oncology research domain — self-experimentation is inappropriate.
  • ·Unknown human safety package.
  • ·Not FDA-approved.
  • ·Pregnancy out of scope.

Catalog match

PNC-27

5mg x 10 vials · from $45

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