Reconstitution
50 mg vial
2S50

SS-31 50 mg

Educational recon guide for a 50 mg SS-31 (elamipretide-class) research vial. Research use only — investigational mitochondria-targeting peptide.

Vial size

Same research phases — calculator defaults and unit math match the selected vial strength.

Vial

50 mg

Your BAC water

5 mL

Concentration

10.00 mg/mL

Per unit

~100.0 mcg

Frequency

Study-design dependent (clinical development often IV infusion; secondary SC tables vary)

Route

Clinical trials often IV; secondary research-vial tables sometimes SC

Live reconstitution calculator

Defaults match this guide (50 mg + 5 mL BAC). Change water or dose and the unit tables below update automatically.

Step 1

Reconstitution

Peptide amount in the vial and how much bacteriostatic water you add.

Bacteriostatic water (mL)
Concentration10 mg/mL
Per 1 unit (0.01 mL)100 mcg
Per 10 units (0.1 mL)1000 mcg

Step 2

Your dose

Quick-select common research doses (includes 2.5 / 7.5 for dual-agonist ladders) or type any mg / mcg value.

Desired dose

Quick select (mg) — switch units or type any value

Insulin syringe (U-100)

Suggested: 50-unit barrel (smallest that fits this draw).

Your draw

Results

Total draw

50 units

Volume

0.500 mL

Doses per vial

10

Syringes needed

1

Barrel

50-unit

Fits one syringe?

Yes

50-unit · 0.5 mL

Filled to 50 u

Fill one 50-unit U-100 syringe to about 50 units (0.500 mL).

Research cycle phases

Units and volumes recompute from your Step 1 concentration (5 mL BAC → 10.00 mg/mL · 100.0 mcg/unit). Doses below are once daily during the course — not weekly.

Standard course

Elamipretide (SS-31) clinical work has used IV infusions (e.g. mg/kg/h over hours) and other parenteral regimens. There is no simple approved SC “wellness” dose. Secondary research-vial tables sometimes discuss multi-milligram SC amounts — treat as recon math only.

Live from Step 1
PhaseDaily doseUnitsVolume
Educational SC · 5 mg5 mg5,000 mcg50 u0.50 mLMulti-mg educational SC illustration
Educational SC · 10 mg10 mg10,000 mcg100 u1.00 mLHigher multi-mg educational illustration

Alternate / higher courses

Shorter or higher daily courses discussed in research communities — not a clinical recommendation

Live from Step 1
PhaseDaily doseUnitsVolume
Educational · 20 mg20 mg20,000 mcg200 u2.00 mLUpper multi-mg illustration — vial-size limited

Supplies planner

Using the guide’s phase ladder (5 mg → 10 mg per injection (phase ladder)) over a 2-day course. Set a dose in Step 2 to plan supplies at your own amount.

Updates with calculator

Your recon

5 mL · 10.00 mg/mL

Dose in plan

Phase ladder

Syringe size

100-unit

Horizon

2-day course

SS-31 50 mg vials

1 vial

~15 mg mg total peptide over this 2-day course at 5 mg → 10 mg per injection (phase ladder). Each vial holds 50 mg.

Bacteriostatic water

2 × 3 mL or 1 × 10 mL

5 mL BAC per vial × 1 vial = 5.0 mL total. Buy as 2× 3 mL bottles or 1× 10 mL bottle (whichever you stock).

U-100 insulin syringes (100-unit)

2+ syringes

About one 100-unit syringe per injection for this schedule. Keep a few spares.

Alcohol swabs & sharps

~6 swabs · 1 sharps container

Wipe vial stopper and injection site each time. Dispose needles in a proper sharps container.

Reconstitution steps

  1. Allow the lyophilized 50 mg SS-31 vial and bacteriostatic water to reach room temperature.
  2. Draw 5.0 mL bacteriostatic water (default for this guide).
  3. Inject BAC slowly down the inner wall of the vial — avoid blasting the powder cake.
  4. Gently swirl or roll until the solution is clear. Do not shake hard (foaming).
  5. Label with recon date and concentration (e.g. "10.0 mg/mL").
  6. Refrigerate at 2–8 °C (35–46 °F), protected from light. Prefer using a reconstituted multi-dose vial within ~28 days with sterile technique.

Storage

Lyophilized

Keep dry powder cold and dark when possible (−20 °C / −4 °F preferred for long-term research stock).

Reconstituted

Refrigerate at 2–8 °C (35–46 °F). Avoid freeze–thaw of the working solution. Prefer finishing within ~28 days.

  • ·Write recon date and BAC volume on the label.
  • ·Protect from light during storage.
  • ·Discard if cloudy, particulates, or odd odor appear after reconstitution.

Protocol notes

  • ·Also known as elamipretide / Bendavia in clinical development literature.
  • ·Not a casual recovery peptide with validated consumer SC charts.
  • ·Research use only.

Research context & considerations

SS-31 targets cardiolipin/mitochondrial membranes and has been studied in mitochondrial myopathy and other indications with mixed late-stage results. Research vials are not approved drugs.

  • ·Investigational — unknown long-term research-vial safety.
  • ·Not FDA-approved as a research-vial consumer product.
  • ·Pregnancy out of scope.

Catalog match

SS·31 (2S50)

50mg x 10 vials · from $130

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