Reconstitution
100 mg vial
AR100

AICAR 100 mg

Educational recon guide for a 100 mg AICAR research vial and daily unit mapping. Research use only.

Vial size

Same research phases — calculator defaults and unit math match the selected vial strength.

Vial

100 mg

Your BAC water

5 mL

Concentration

20.00 mg/mL

Per unit

~200.0 mcg

Frequency

Once daily (some write-ups 5 days on / 2 off)

Route

Subcutaneous / IM in secondary research discussions

Live reconstitution calculator

Defaults match this guide (100 mg + 5 mL BAC). Change water or dose and the unit tables below update automatically.

Step 1

Reconstitution

Peptide amount in the vial and how much bacteriostatic water you add.

Bacteriostatic water (mL)
Concentration20 mg/mL
Per 1 unit (0.01 mL)200 mcg
Per 10 units (0.1 mL)2000 mcg

Step 2

Your dose

Quick-select common research doses (includes 2.5 / 7.5 for dual-agonist ladders) or type any mg / mcg value.

Desired dose

Quick select (mg) — switch units or type any value

Insulin syringe (U-100)

Suggested: 30-unit barrel (smallest that fits this draw).

Your draw

Results

Total draw

5 units

Volume

0.050 mL

Doses per vial

100

Syringes needed

1

Barrel

30-unit

Fits one syringe?

Yes

30-unit · 0.3 mL

Filled to 5 u

Fill one 30-unit U-100 syringe to about 5 units (0.050 mL).

Research cycle phases

Units and volumes recompute from your Step 1 concentration (5 mL BAC → 20.00 mg/mL · 200.0 mcg/unit). Doses below are once daily during the course — not weekly.

Standard course

Vendor/community charts often show ~1–5 mg/day; animal literature uses large mg/kg ranges — do not scale those naively.

Live from Step 1
PhaseDaily doseUnitsVolume
Days 1–7 (intro)1 mg1,000 mcg5 u0.05 mL
Days 8–28 (standard educational)3 mg3,000 mcg15 u0.15 mL
Days 29–42 (optional)5 mg5,000 mcg25 u0.25 mL

Alternate / higher courses

Shorter or higher daily courses discussed in research communities — not a clinical recommendation

Live from Step 1
PhaseDaily doseUnitsVolume
Higher educational · 10 mg daily × 2810 mg10,000 mcg50 u0.50 mL
Higher educational · 25 mg daily × 1425 mg25,000 mcg125 u1.25 mLVolume >1.0 mL — split across syringes/sites

Supplies planner

Using the guide’s phase ladder (1 mg → 3 mg → 5 mg per injection (phase ladder)) over a 42-day course. Set a dose in Step 2 to plan supplies at your own amount.

Updates with calculator

Your recon

5 mL · 20.00 mg/mL

Dose in plan

Phase ladder

Syringe size

100-unit

Horizon

42-day course

AICAR 100 mg vials

2 vials

~140 mg mg total peptide over this 42-day course at 1 mg → 3 mg → 5 mg per injection (phase ladder). Each vial holds 100 mg.

Bacteriostatic water

4 × 3 mL or 1 × 10 mL

5 mL BAC per vial × 2 vials = 10.0 mL total. Buy as 4× 3 mL bottles or 1× 10 mL bottle (whichever you stock).

U-100 insulin syringes (100-unit)

42+ syringes

About one 100-unit syringe per injection for this schedule. Keep a few spares.

Alcohol swabs & sharps

~88 swabs · 1 sharps container

Wipe vial stopper and injection site each time. Dispose needles in a proper sharps container.

Reconstitution steps

  1. Allow the lyophilized 100 mg AICAR vial and bacteriostatic water to reach room temperature.
  2. Draw 5.0 mL bacteriostatic water (default for this guide).
  3. Inject BAC slowly down the inner wall of the vial — avoid blasting the powder cake.
  4. Gently swirl or roll until the solution is clear. Do not shake hard (foaming).
  5. Label with recon date and concentration (e.g. "20.0 mg/mL — 5 mL BAC").
  6. Refrigerate at 2–8 °C (35–46 °F), protected from light. Prefer using a reconstituted multi-dose vial within ~28 days with sterile technique.

Storage

Lyophilized

Keep dry powder cold and dark when possible (−20 °C / −4 °F preferred for long-term research stock).

Reconstituted

Refrigerate at 2–8 °C (35–46 °F). Avoid freeze–thaw of the working solution. Prefer finishing within ~28 days.

  • ·Write recon date and BAC volume on the label.
  • ·Protect from light during storage.
  • ·Discard if cloudy, particulates, or odd odor appear after reconstitution.

Protocol notes

  • ·AICAR is an AMPK pathway research tool.
  • ·Animal mg/kg doses are not naive human schedules.
  • ·Research use only.

Research context & considerations

AICAR is studied as an AMPK activator in metabolic and exercise-physiology models.

  • ·Not FDA-approved for exercise enhancement or fat loss.
  • ·Pregnancy and pediatric use out of scope.

Catalog match

Aicar (AR100)

100mg x 10 vials · from $50

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