AICAR 50 mg
Educational recon guide for a 50 mg AICAR research vial and daily unit mapping. Research use only.
Vial size
Same research phases — calculator defaults and unit math match the selected vial strength.
Vial
50 mg
Your BAC water
3 mL
Concentration
16.67 mg/mL
Per unit
~166.7 mcg
Frequency
Once daily (some write-ups 5 days on / 2 off)
Route
Subcutaneous / IM in secondary research discussions
Live reconstitution calculator
Defaults match this guide (50 mg + 3 mL BAC). Change water or dose and the unit tables below update automatically.
Step 1
Reconstitution
Peptide amount in the vial and how much bacteriostatic water you add.
Step 2
Your dose
Quick-select common research doses (includes 2.5 / 7.5 for dual-agonist ladders) or type any mg / mcg value.
Quick select (mg) — switch units or type any value
Suggested: 30-unit barrel (smallest that fits this draw).
Your draw
Results
Total draw
6 units
Volume
0.060 mL
Doses per vial
50
Syringes needed
1
Barrel
30-unit
Fits one syringe?
Yes
30-unit · 0.3 mL
Filled to 6 u
Fill one 30-unit U-100 syringe to about 6 units (0.060 mL).
Research cycle phases
Units and volumes recompute from your Step 1 concentration (3 mL BAC → 16.67 mg/mL · 166.7 mcg/unit). Doses below are once daily during the course — not weekly.
Standard course
Vendor/community charts often show ~1–5 mg/day; animal literature uses large mg/kg ranges — do not scale those naively.
| Phase | Daily dose | Units | Volume |
|---|---|---|---|
| Days 1–7 (intro) | 1 mg1,000 mcg | 6.0 u | 0.06 mL |
| Days 8–28 (standard educational) | 3 mg3,000 mcg | 18.0 u | 0.18 mL |
| Days 29–42 (optional) | 5 mg5,000 mcg | 30.0 u | 0.30 mL |
Alternate / higher courses
Shorter or higher daily courses discussed in research communities — not a clinical recommendation
| Phase | Daily dose | Units | Volume |
|---|---|---|---|
| Higher educational · 10 mg daily × 28 | 10 mg10,000 mcg | 60.0 u | 0.60 mLPrefer 100-unit syringe |
| Higher educational · 25 mg daily × 14 | 25 mg25,000 mcg | 150.0 u | 1.50 mLVolume >1.0 mL — split across syringes/sites |
Supplies planner
Using the guide’s phase ladder (1 mg → 3 mg → 5 mg per injection (phase ladder)) over a 42-day course. Set a dose in Step 2 to plan supplies at your own amount.
Your recon
3 mL · 16.67 mg/mL
Dose in plan
Phase ladder
Syringe size
100-unit
Horizon
42-day course
AICAR 50 mg vials
3 vials
~140 mg mg total peptide over this 42-day course at 1 mg → 3 mg → 5 mg per injection (phase ladder). Each vial holds 50 mg.
Bacteriostatic water
3 × 3 mL or 1 × 10 mL
3 mL BAC per vial × 3 vials = 9.0 mL total. Buy as 3× 3 mL bottles or 1× 10 mL bottle (whichever you stock).
U-100 insulin syringes (100-unit)
42+ syringes
About one 100-unit syringe per injection for this schedule. Keep a few spares.
Alcohol swabs & sharps
~90 swabs · 1 sharps container
Wipe vial stopper and injection site each time. Dispose needles in a proper sharps container.
Reconstitution steps
- Allow the lyophilized 50 mg AICAR vial and bacteriostatic water to reach room temperature.
- Draw 3.0 mL bacteriostatic water (default for this guide).
- Inject BAC slowly down the inner wall of the vial — avoid blasting the powder cake.
- Gently swirl or roll until the solution is clear. Do not shake hard (foaming).
- Label with recon date and concentration (e.g. "16.7 mg/mL — 3 mL BAC").
- Refrigerate at 2–8 °C (35–46 °F), protected from light. Prefer using a reconstituted multi-dose vial within ~28 days with sterile technique.
Storage
Lyophilized
Keep dry powder cold and dark when possible (−20 °C / −4 °F preferred for long-term research stock).
Reconstituted
Refrigerate at 2–8 °C (35–46 °F). Avoid freeze–thaw of the working solution. Prefer finishing within ~28 days.
- ·Write recon date and BAC volume on the label.
- ·Protect from light during storage.
- ·Discard if cloudy, particulates, or odd odor appear after reconstitution.
Protocol notes
- ·AICAR is an AMPK pathway research tool.
- ·Animal mg/kg doses are not naive human schedules.
- ·Research use only.
Research context & considerations
AICAR is studied as an AMPK activator in metabolic and exercise-physiology models.
- ·Not FDA-approved for exercise enhancement or fat loss.
- ·Pregnancy and pediatric use out of scope.
Catalog match
Aicar (AR50)
50mg x 10 vials · from $30