Reconstitution
5 mg vial
BT5

TB-500 5 mg

Educational recon guide for a 5 mg TB-500 research vial and multi-mg loading/maintenance unit mapping. Research use only.

Vial size

Same research phases — calculator defaults and unit math match the selected vial strength.

Vial

5 mg

Your BAC water

2 mL

Concentration

2.50 mg/mL

Per unit

~25.0 mcg

Frequency

Loading often 2× weekly multi-mg, then lower weekly maintenance in secondary write-ups

Route

Subcutaneous (sometimes near injury site in community discussions)

Live reconstitution calculator

Defaults match this guide (5 mg + 2 mL BAC). Change water or dose and the unit tables below update automatically.

Step 1

Reconstitution

Peptide amount in the vial and how much bacteriostatic water you add.

Bacteriostatic water (mL)
Concentration2.50 mg/mL
Per 1 unit (0.01 mL)25 mcg
Per 10 units (0.1 mL)250 mcg

Step 2

Your dose

Quick-select common research doses (includes 2.5 / 7.5 for dual-agonist ladders) or type any mg / mcg value.

Desired dose

Quick select (mg) — switch units or type any value

Insulin syringe (U-100)

Suggested: 100-unit barrel (smallest that fits this draw).

Your draw

Results

Total draw

80 units

Volume

0.800 mL

Doses per vial

2.5

Syringes needed

1

Barrel

100-unit

Fits one syringe?

Yes

100-unit · 1.0 mL

Filled to 80 u

Fill one 100-unit U-100 syringe to about 80 units (0.800 mL).

Weekly research phases

Units and volumes recompute from your Step 1 concentration (2 mL BAC → 2.50 mg/mL · 25.0 mcg/unit).

Standard titration

Secondary educational tables commonly load roughly ~2–5 mg per injection, 2× weekly for several weeks (≈4–10 mg/week), then reduce to lower weekly or less-frequent maintenance. Not an FDA-approved drug.

Live from Step 1
PhaseWeekly doseUnitsVolume
Load · 2 mg per injection2 mg2,000 mcg80 u0.80 mLCommon lower load amount (often 2×/week)
Load · 2.5 mg per injection2.50 mg2,500 mcg100 u1.00 mLCommon mid load amount
Load · 5 mg per injection5 mg5,000 mcg200 u2.00 mLUpper common load amount

Advanced / higher-end

Faster escalation discussed in research communities — not a clinical recommendation

Live from Step 1
PhaseWeekly doseUnitsVolume
Maintenance illustration · 2 mg weekly2 mg2,000 mcg80 u0.80 mLLower weekly maintenance-style amount after load

Supplies planner

Using the guide’s phase ladder (2 mg → 2.5 mg → 5 mg weekly (phase ladder)) over a 12-week schedule. Set a dose in Step 2 to plan supplies at your own amount.

Updates with calculator

Your recon

2 mL · 2.50 mg/mL

Dose in plan

Phase ladder

Syringe size

100-unit

Horizon

12-week schedule

TB-500 5 mg vials

8 vials

~38 mg mg total peptide over this 12-week schedule at 2 mg → 2.5 mg → 5 mg weekly (phase ladder). Each vial holds 5 mg.

Bacteriostatic water

6 × 3 mL or 2 × 10 mL

2 mL BAC per vial × 8 vials = 16.0 mL total. Buy as 6× 3 mL bottles or 2× 10 mL bottles (whichever you stock).

U-100 insulin syringes (100-unit)

16+ syringes

Some doses need more than one 100-unit syringe at your concentration — plan extras and split draws when the math exceeds the barrel.

Alcohol swabs & sharps

~48 swabs · 1 sharps container

Wipe vial stopper and injection site each time. Dispose needles in a proper sharps container.

Reconstitution steps

  1. Allow the lyophilized 5 mg TB-500 vial and bacteriostatic water to reach room temperature.
  2. Draw 2.0 mL bacteriostatic water (default for this guide).
  3. Inject BAC slowly down the inner wall of the vial — avoid blasting the powder cake.
  4. Gently swirl or roll until the solution is clear. Do not shake hard (foaming).
  5. Label with recon date and concentration (e.g. "2.5 mg/mL").
  6. Refrigerate at 2–8 °C (35–46 °F), protected from light. Prefer using a reconstituted multi-dose vial within ~28 days with sterile technique.

Storage

Lyophilized

Keep dry powder cold and dark when possible (−20 °C / −4 °F preferred for long-term research stock).

Reconstituted

Refrigerate at 2–8 °C (35–46 °F). Avoid freeze–thaw of the working solution. Prefer finishing within ~28 days.

  • ·Write recon date and BAC volume on the label.
  • ·Protect from light during storage.
  • ·Discard if cloudy, particulates, or odd odor appear after reconstitution.

Protocol notes

  • ·Often discussed with BPC-157 (see blend guides).
  • ·Research use only.

Research context & considerations

TB-500 is a synthetic fragment related to thymosin β4 research on cell migration and tissue repair. Evidence is largely preclinical/anecdotal relative to approved medicines.

  • ·Theoretical angiogenesis/cancer-growth caution discussions exist for the class.
  • ·Not FDA-approved.
  • ·Pregnancy out of scope.

Catalog match

TB500 (BT5)

5mg x 10 vials · from $25

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