Reconstitution
5 mg vial
RT5

Retatrutide 5 mg

Educational guide for reconstituting a 5 mg Retatrutide research vial and mapping weekly doses to U-100 units. Research use only.

Vial size

Same research phases — calculator defaults and unit math match the selected vial strength.

Vial

5 mg

Your BAC water

1 mL

Concentration

5.00 mg/mL

Per unit

~50.0 mcg

Frequency

Once weekly (same weekday)

Route

Subcutaneous

Live reconstitution calculator

Defaults match this guide (5 mg + 1 mL BAC). Change water or dose and the unit tables below update automatically.

Step 1

Reconstitution

Peptide amount in the vial and how much bacteriostatic water you add.

Bacteriostatic water (mL)
Concentration5 mg/mL
Per 1 unit (0.01 mL)50 mcg
Per 10 units (0.1 mL)500 mcg

Step 2

Your dose

Quick-select common research doses (includes 2.5 / 7.5 for dual-agonist ladders) or type any mg / mcg value.

Desired dose

Quick select (mg) — switch units or type any value

Insulin syringe (U-100)

Suggested: 50-unit barrel (smallest that fits this draw).

Your draw

Results

Total draw

40 units

Volume

0.400 mL

Doses per vial

2.5

Syringes needed

1

Barrel

50-unit

Fits one syringe?

Yes

50-unit · 0.5 mL

Filled to 40 u

Fill one 50-unit U-100 syringe to about 40 units (0.400 mL).

Weekly research phases

Units and volumes recompute from your Step 1 concentration (1 mL BAC → 5.00 mg/mL · 50.0 mcg/unit).

Standard titration

Research-community tables often start ~2 mg weekly and titrate toward ~4–8+ mg. Not an approved drug product; trial designs differ.

Live from Step 1
PhaseWeekly doseUnitsVolume
Weeks 1–42 mg2,000 mcg40 u0.40 mLIntroductory research-community amount
Weeks 5–84 mg4,000 mcg80 u0.80 mLPrefer 100-unit syringe
Weeks 9–126 mg6,000 mcg120 u1.20 mLVolume >1.0 mL — split across two injection sites
Weeks 13+8 mg8,000 mcg160 u1.60 mLCommon maintenance discussion range

Advanced / higher-end

Faster escalation discussed in research communities — not a clinical recommendation

Live from Step 1
PhaseWeekly doseUnitsVolume
Aggressive · Weeks 1–44 mg4,000 mcg80 u0.80 mLPrefer 100-unit syringe
Aggressive · Weeks 5–88 mg8,000 mcg160 u1.60 mLVolume >1.0 mL — split across two injection sites
Aggressive · Weeks 9–1210 mg10,000 mcg200 u2.00 mLUpper educational range — check syringe capacity

Supplies planner

Using the guide’s phase ladder (2 mg → 4 mg → 6 mg weekly (phase ladder)) over a 12-week schedule. Set a dose in Step 2 to plan supplies at your own amount.

Updates with calculator

Your recon

1 mL · 5.00 mg/mL

Dose in plan

Phase ladder

Syringe size

100-unit

Horizon

12-week schedule

Retatrutide 5 mg vials

10 vials

~48 mg mg total peptide over this 12-week schedule at 2 mg → 4 mg → 6 mg weekly (phase ladder). Each vial holds 5 mg.

Bacteriostatic water

4 × 3 mL or 1 × 10 mL

1 mL BAC per vial × 10 vials = 10.0 mL total. Buy as 4× 3 mL bottles or 1× 10 mL bottle (whichever you stock).

U-100 insulin syringes (100-unit)

16+ syringes

Some doses need more than one 100-unit syringe at your concentration — plan extras and split draws when the math exceeds the barrel.

Alcohol swabs & sharps

~52 swabs · 1 sharps container

Wipe vial stopper and injection site each time. Dispose needles in a proper sharps container.

Reconstitution steps

  1. Allow the lyophilized 5 mg vial and bacteriostatic water to reach room temperature.
  2. Draw 1.0 mL bacteriostatic water (default).
  3. Inject BAC slowly down the inner wall of the vial — avoid blasting the powder cake.
  4. Gently swirl or roll until the solution is clear. Do not shake hard (foaming).
  5. Label with recon date and concentration (e.g. “5.0 mg/mL”).
  6. Refrigerate at 2–8 °C (35–46 °F), protected from light.

Storage

Lyophilized

Keep dry powder cold and dark (−20 °C / −4 °F preferred for long-term research stock).

Reconstituted

Refrigerate at 2–8 °C (35–46 °F). Avoid freeze–thaw. Prefer using within ~28 days with sterile technique.

  • ·Write recon date and BAC volume on the label.
  • ·Protect from light.
  • ·Discard if cloudy, particulates, or odd odor appear.

Protocol notes

  • ·Weekly subcutaneous research framing — not daily.
  • ·Titrate slowly; GI-class effects are commonly discussed for multi-agonist incretin research peptides.
  • ·Rotate injection sites.
  • ·Research use only. Not medical advice.

Research context & considerations

Retatrutide is an investigational triple agonist (GLP-1 / GIP / glucagon pathways) studied for obesity and metabolic outcomes. Research-market vials are not the same as clinical trial drug product and are not FDA-approved for consumer use.

  • ·GI effects (nausea, etc.) are commonly discussed for the class.
  • ·Not for pregnancy, breastfeeding, or pediatric use.
  • ·Personal/family medullary thyroid carcinoma and MEN2 are classic GLP-1-class caution contexts in approved-drug labels — relevant as research caution framing.
  • ·Not a substitute for licensed medical care.

Catalog match

Retatrutide (RT5)

5mg x 10 vials · from $35

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