Reconstitution
2 mg vial
SM2

Semaglutide 2 mg

Educational recon guide for a 2 mg Semaglutide research vial and once-weekly unit mapping. Research use only — not Ozempic/Wegovy pens.

Vial size

Same research phases — calculator defaults and unit math match the selected vial strength.

Vial

2 mg

Your BAC water

1 mL

Concentration

2.00 mg/mL

Per unit

~20.0 mcg

Frequency

Once weekly subcutaneous (same weekday)

Route

Subcutaneous

Live reconstitution calculator

Defaults match this guide (2 mg + 1 mL BAC). Change water or dose and the unit tables below update automatically.

Step 1

Reconstitution

Peptide amount in the vial and how much bacteriostatic water you add.

Bacteriostatic water (mL)
Concentration2 mg/mL
Per 1 unit (0.01 mL)20 mcg
Per 10 units (0.1 mL)200 mcg

Step 2

Your dose

This guide uses microgram-scale research doses — quick picks are in mcg (100 mcg = 0.1 mg).

Desired dose

Quick select (mcg) — switch units or type any value

Insulin syringe (U-100)

Suggested: 30-unit barrel (smallest that fits this draw).

Your draw

Results

Total draw

12.5 units

Volume

0.125 mL

Doses per vial

8

Syringes needed

1

Barrel

30-unit

Fits one syringe?

Yes

30-unit · 0.3 mL

Filled to 12.5 u

Fill one 30-unit U-100 syringe to about 12.5 units (0.125 mL).

Weekly research phases

Units and volumes recompute from your Step 1 concentration (1 mL BAC → 2.00 mg/mL · 20.0 mcg/unit).

Standard titration

Approved chronic weight-management product (Wegovy) titrates 0.25 → 0.5 → 1.0 → 1.7 → 2.4 mg once weekly if tolerated. Diabetes products (Ozempic) commonly use lower weekly ceilings. Research powders are not branded pens.

Live from Step 1
PhaseWeekly doseUnitsVolume
Weeks 1–4 · 0.25 mg250 mcg0.25 mg12.5 u0.13 mLEducational start
Weeks 5–8 · 0.5 mg500 mcg0.5 mg25 u0.25 mL
Weeks 9–12 · 1.0 mg1 mg1,000 mcg50 u0.50 mL
Weeks 13–16 · 1.7 mg1.70 mg1,700 mcg85 u0.85 mLPrefer 100-unit syringe
Weeks 17+ · 2.4 mg2.40 mg2,400 mcg120 u1.20 mLWegovy-style maintenance educational target

Advanced / higher-end

Faster escalation discussed in research communities — not a clinical recommendation

Live from Step 1
PhaseWeekly doseUnitsVolume
Hold at 1.0 mg weekly1 mg1,000 mcg50 u0.50 mLIf higher steps not tolerated / diabetes-product style ceiling discussions
Higher educational · 2.0 mg weekly2 mg2,000 mcg100 u1.00 mLSome product labels use 2.0 mg weekly as a step

Supplies planner

Using the guide’s phase ladder (250 mcg → 500 mcg → 1 mg weekly (phase ladder)) over a 12-week schedule. Set a dose in Step 2 to plan supplies at your own amount.

Updates with calculator

Your recon

1 mL · 2.00 mg/mL

Dose in plan

Phase ladder

Syringe size

100-unit

Horizon

12-week schedule

Semaglutide 2 mg vials

4 vials

~7 mg mg total peptide over this 12-week schedule at 250 mcg → 500 mcg → 1 mg weekly (phase ladder). Each vial holds 2 mg.

Bacteriostatic water

2 × 3 mL or 1 × 10 mL

1 mL BAC per vial × 4 vials = 4.0 mL total. Buy as 2× 3 mL bottles or 1× 10 mL bottle (whichever you stock).

U-100 insulin syringes (100-unit)

12+ syringes

About one 100-unit syringe per injection for this schedule. Keep a few spares.

Alcohol swabs & sharps

~32 swabs · 1 sharps container

Wipe vial stopper and injection site each time. Dispose needles in a proper sharps container.

Reconstitution steps

  1. Allow the lyophilized 2 mg Semaglutide vial and bacteriostatic water to reach room temperature.
  2. Draw 1.0 mL bacteriostatic water (default for this guide).
  3. Inject BAC slowly down the inner wall of the vial — avoid blasting the powder cake.
  4. Gently swirl or roll until the solution is clear. Do not shake hard (foaming).
  5. Label with recon date and concentration (e.g. "2.0 mg/mL").
  6. Refrigerate at 2–8 °C (35–46 °F), protected from light. Prefer using a reconstituted multi-dose vial within ~28 days with sterile technique.

Storage

Lyophilized

Keep dry powder cold and dark when possible (−20 °C / −4 °F preferred for long-term research stock).

Reconstituted

Refrigerate at 2–8 °C (35–46 °F). Avoid freeze–thaw of the working solution. Prefer finishing within ~28 days.

  • ·Write recon date and BAC volume on the label.
  • ·Protect from light during storage.
  • ·Discard if cloudy, particulates, or odd odor appear after reconstitution.

Protocol notes

  • ·Once-weekly GLP-1 agonist — titrate slowly for GI tolerability.
  • ·Research powders ≠ Ozempic/Wegovy pens.
  • ·Research use only.

Research context & considerations

Semaglutide is an approved GLP-1 receptor agonist for T2D and chronic weight management as branded products. Catalog research vials are a separate unregulated channel.

  • ·GI effects common during titration.
  • ·GLP-1-class label cautions apply (gallbladder, pancreatitis discussions, MTC/MEN2 framing on approved products).
  • ·Not FDA-approved as a research-vial consumer product.
  • ·Pregnancy out of scope.

Catalog match

Semaglutide (SM2)

2mg x 10 vials · from $20

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